Explore Microbes at Home

 

 

About replace material of this project

 

Dear Dr.Michelle

I got a microbes kit but I have done many projects and the easygels ran off. Is there any other material that I can use?
Can I use gelatin? But if I use gelatin, how can I know whether there is bacteria? Is there anything that I can stain them?

Please reply as soon as possible, Thankyou

sciangel

 

Re: About replace material of this project

 

If you use gelatin, you will need to add a nutritive substance like a beef or chicken bouillon cube and sugar. There are various recipes online. However many bacteria poduce enzymes that can dissolve and liquify gelatin, which is made of protein itself. So agar is better. It is not subject to this problem. You do not need to stain the bacteria to see them. They will appear as white or yellowish dots on the surface.

Dr. Michelle

Re: About replace material of this project

 

You can now buy a replacement set of plates and media at:

http://www.1shoppingcart.com/SecureCart/SecureCart.aspx?mid=FA8D15FC-AE8B-4432-A0F0-3AA8501B2F48&pid=339593a73c72dc242bb75ff586d7384a&bn=1

 

 



 

If the bacteria keep increasing, is it just growing, or is i

 

If the bacteria keep increasing, is it just growing, or is it the original bacteria that was in the water?

 

Re: If the bacteria keep increasing, is it just growing, or is i

ostby DrMichelle on Wed Aug 26, 2009 3:59 pm

E. coli and other coliforms grow very rapidly when compared to most other bacteria, so they generally appear in the plate first. Any colonies that appear after 48 hours should be disregarded.

Second, if you are incubating at lower temperature ("room temp"), the colonies will not appear as soon. In this case, you should wait until you are seeing the first colored colonies and then give at least another 18-20 hours for further growth and development of color before counting the results.

Colonies that do not grow past a pin point size (less than 0.25 mm) within 48 hrs at 35 C. should not be counted without further testing, especially if they were late showing up. An exception to this rule is if there are so many colonies in the plate that it is covered with colonies very close together. Such a plate can even look like it doesnŐt have any colonies at all and just have a pink or bluish cast throughout the whole plate.

Colonies growing down in the solid medium will be smaller than those growing on the surface and surface colonies generally are paler in color. Also, if colonies are crowded close to each other on/in the medium, they will not grow as large.

Rarely, there may be present a large colored spot growing on the surface or under the medium on the plate. This is usually the result of a colony growing at the edge of the plate and down under the medium where there may be excessive moisture where it can spread, and it should be counted as only one colony forming unit.

Dr. Michelle

 

Top



 

 

Why do the bacteria appear red?


Why do the bacteria appear red?

Suzan

Re: Why do the bacteria appear red?

 

There is a Tetrazolium salt added to the petri dish that reacts with an enzyme made only by living cells, that turns the salt pink.
Dr. Michelle

 


What is the Easygel made of?

 

Dr. Michelle,
What is the Easygel made of?

Erica

Re: What is the Easygel made of?

 

Erica,

Easygel is a pectin based bacterial growth media that gels when it comes in contact with the specially treated petri dish.

Dr. Michelle

 

 

Delay between collection and plating

 

Dear Dr. Shawn,

I am collecting a sample at the local animal shelter to collect saliva. It takes 45 minutes to drive back home. What would be the most efficient way to collect the sample: dip the applicator into the easygel & then pour into petri dish there or just leave it in the easygel bottle (w/the lid securely back on) & pour it into the petri dish when we get back home?
Thanks for your help!!!

--
Leslie Anne

 

Re: delay between collection and plating

 

Leslie,

That's a good question. If you have access to a clean flat surface, you could pour the plates there. However, it takes about 45 minutes for the gel to solidify once it is in the plates and so you really don't want to move them for at least this long, once they are poured.

I think it would be safer to dip the applicator into the easygel bottle, swish it around, then remove it before promptly capping the bottle. You can then pour the liquid into the plates when you get home. The Easygel will remain a liquid until it's combined with the plate, which is treated at the bottom. If you want to make sure no bacterial growth occurs until you get home, you can put your bottles into a cooler.

Let us know how your experiment goes!

Dr. Michelle


 

 

Some question about 'microbe incubator'

 

Dear Dr. Michelle,

I am the one who ask a question here last time! The problem finally was solved as I find that there are still 5 easygel left. Anyway, thanks your support.
This time, I have some question about the 'microbe incubator' in the instruction booklet! I don't quite understand how to do that...... Also, I want to ask
if I can get a copy of the instruction booklet, as I have make some 'dirt' on mine...And I am not a member of scifair.org as this kit is presented from my friends...
If you can, please send it to my email, i'll keep it well! Thanks

Yours,
Angel

 


 

Is Easygel safe?

 

Is easygel safe?

 

Re: Is Easygel safe?

 

Yes, it's very safe. It's just food for bacteria and starts off
completely sterile. I wouldn't recommend drinking it however.

Dr. Michelle

 


Microbes not growing

 

We tested a toilet seat, money and a cell phone and only one spot is
growing
on one dish after 48 hours! The petri dishes are in a drawer in a
bedroom
at a fairly constant temp.
Help!

We tested 1" square areas of each.

Thanks,
Brigette

Re: Microbes not growing

 

You might give your dishes one more day because some bacteria take longer
than others. However, I think that you have discovered
that these surfaces really DON'T contain that many bacteria. In fact some of
the places people would think would be the dirtiest are not. Studies have shown
that toilet seats, contrary to what most people would think, are one
of the cleanest places. Bacteria do not live for long on many
surfaces, especially if they are dry. Places
that are likely to contain a lot more bacteria are kitchen
surfaces, bathroom counters and faucets, dish rags, refrigerator
interior surfaces, and human hands, any place that is not left
untouched for very long. You might want to test some of thes surfaces to
round out your experiment. Try a person's hand (before washing) or
some areas around your kitchen sink.

I believe your experiment has worked perfectly.

Sincerely,

Dr. Michelle

 

Re: Microbes not growing

 

Hello,
We do have a lot more growth at the 3-4 day mark. Just today the little red
specks appeared and I realized what we were seeing may not even be what we
are supposed to report. We had one red spot early on, that was red on the
underneath and was white an fuzzy on the other side. Most of the petri
dishes have white spots. I was rereading the info and found that we are to
only count the red spots...is that so? And if so, what are the other white
spots? We were so excited that they were growing, and now we realize that
they aren't red!

SO, just tonight I noticed what looked like red marker spots that appeared
today. Is THAT what we are supposed to report? And, how long is too long
to grow a sample. Is there a cut off?

Thanks,
Brigette

 

Re: Microbes not growing

 

Hi Brigette,

The colonies of bacteria should be very tiny red dots and grow to be no larger than the size of pin or pencil head.

White spots are most likely mold.

Dr. Michelle

 

Re: Microbes not growing

 

Thanks! By the way, the white spots are moldy spots. I am guessing that
they are dead or non-viable cells...I will include a couple of pictures.
Our project is due Tuesday morning...I think that we are okay because the
red dots finally showed up!!!!!!!! They must be weak strains, because they
took days to form. We did what you said and rounded it off with unwashed
hands...wow, those grew in 24 hours, must be srong strains!

The 3 petri dishes with labels had those moldy spots early on, then the red
dots appeared,(this pic was at 96 hours). There are 2 red dots on the cell
phone and one on the toilet seat. There were none on the dollar bill,
probably because it was not in circulation for months. The dishes labeled L
and R are of the dirty hands and really did not grow the moldy spots (this
pic was around at 65 hours).

Thanks,
Brigette

Attachments

mages of Dishes2.jpg

Images of Dishes2.jpg (53.35 KiB) Viewed 600 times

mages of Dishes1.jpg

Images of Dishes1.jpg (56.9 KiB) Viewed 602 times

 


 

Testing mouths for bacteria

 

Hi there...

My son and I are working on his science fair project... We also recently received your "Home Microbiology" kit...

In a nutshell, we are going to examine whether various mouthrinses can/will reduce the number of germs in our mouths following their use. The protocol is to have a "control" sample taken without the use of a mouthrinse, plus 2-3 samples taken following the use of different mouthrinses on 2-3 other mornings... We plan on using the mouthrinse for 1 minute, swabbing the oral mucosa, and then "inoculating" the EasyGel with the swab before pouring the EasyGel into the petri dish.

Will we be fine simply swabbing the oral mucosa with the dry/sterile swab and then inserting the swab into a bottle of EasyGel to inoculate it? Or should the swab the "dampened" with something before swabbing the tissues of the mouth?

Thanks...

Mike

 

Re: Testing mouths for bacteria

 

Mike,

Yes, just use the dry sterile swab to swipe inside the mouth. They'll be plenty of moisture there. You only need to dampen the swab when you are swiping a dry surface. Although the Easygel media is not hazardous, it's not a good idea to put it inside your mouth.

Dr. Michelle

 

 


 

Extract DNA at Home


 

DNA fingerprinting

ostby DrMichelle on Mon Feb 23, 2009 4:17 pm

Hi,

We downloaded you science fair information on electrophoresis. We were wondering if DNA fingerprinting is what is happening during the electrophoresis process?

Thanks,

The Sutherlands

 

Re: DNA fingerprinting

 

Hi,

No, the typical procedure for DNA fingerprinting involves chopping up genomic DNA (the DNA from a cell's nucleus) at key places in the DNA sequence using enzymes called restriction enzymes. One does this to make it possible to look for small length differences in these DNA segments that naturally occur in different people in the population. These pieces can then be sorted by size and analyzed using a number of different methods.

Electrophoresis is the general procedure of using the naturally occurring charge on various molecules; dyes, proteins, DNA, etc. in order to sort them by size. A voltage is applied across the gel and your samples placed at one end. The gel is made of a special material, usually agarose or some other polymer, which forms a uniform matrix that impedes the motion of the molecules. Longer ones take more time to get through the matrix than shorter ones just like it would take longer for large impurities to make it through a column of sand than it would for water to get through. Since it takes longer for large molecules to get through the gel than shorter ones, this method can be used to sort molecules by size, including DNA.

Although electrophoresis is sometimes used to analyze the size of the DNA segments from DNA fingerprinting, discerning the small differences in fragment size requires techniques and materials currently beyond what be used at home.

Hope this helps.

Dr. Michelle

 


Extracting human DNA

 

Dr. Shawn,
Regarding my CSI order. I am very excited to receive it. I am ordering it for a student of mine who is obsessed with doing her science fair project on DNA fingerprinting. She was hoping she could work with hair. (she wanted to see if there is a difference between identical twins, fraturnal twins , ordinary siblings, and non family members.) We were having a hard time getting a recipe for Hair DNA extraction.)

Does it have materials and info for DNA extraction from hair?

Regina

Re: Extracting DNA from humans

 

Dear Regina,

Delighted in your interest with our kit--however.... You can not do DNA fingerprinting this this or any other commercially available kit. The chemicals required to do this work are dangerous and expensive, and the method of separating the strands of DNA is also expensive and uses dangerous chemicals. So you won't find a kit that will enable you to test one human's DNA against another that is intended for science fair students.

You don't want to extract DNA from hair because only the living cells at the root of the hair contain DNA, so you would need a lot of hair to get enough DNA to see. Scientists use a method called Polymerase Chain Reaction or PCR to amplify small amounts of DNA in the amounts that can be analyzed. This takes special equipment and enzymes. The kit extracts DNA from living plants and from animal tissue, like things you find in your produce and meat department at your store.

That being said, our kit will allow your student to extract DNA hundreds of times from all kinds of things in the living world and to do a number of experiments with it.

Yours for great science,

Dr. Shawn

 


 

DNA from banana in different states of ripeness

 

Dear Sir:

My son is doing a science project using your DNA Kit and I have a few questions so I will know if his hypothesis will be correct or not and what a proper conclusion can be written. He extracted DNA from an underripe, ripe, and overripe banana. His hypothesis states that he thinks the ripe banana will contain the most extractable and measurable DNA. Should it?
Also, how do you measure the amount of DNA -is it the blue band only or what lies in between. We are not sure. It looks like 3 ml for the underripe banana, 2 ml for the ripe banana and 1 ml for the overripe banana. Do these measurements sound correct to you.
Please advise as soon as possible. Thank you.

 

Re: DNA from banana in different states of ripeness

 

There are several possibilities for why the quantity of DNA extracted will be different for fruit in different states of ripeness.

One reason is that the density of cells could be higher for the fruit in various states of ripeness. In other words, more cells for a given volume of fruit gives you more DNA. This is because, the amount of DNA in the nucleus in a given cell type will be exactly the same for all its cells. If a fruit's cells are really big because they contain a lot of water or other stuff, not as many cells will fit into a given space, and so you'll have less DNA.

Another reason could be that the ability to get the DNA out of the cells changes in each of the different states of ripeness. It could be that it's harder to get the DNA out of underipe cells, for example because they have thicker cell walls.

A reason why ripe fruit would give you less DNA would be that in very ripe fruit, many enzymes are released during the ripening process which can break down the proteins and DNA in the cells.

There can be other reasons as well. When scientists do experiments like this they usually get more questions than answers and have to do more experiments to answer the new questions. I would suggest that you give some of the different reasons why you got the results that you did and then maybe suggest an experiment that someone could do in the future to rule out one of these hypotheses. I don't know the actual answer but you certainly have a reasonable result and can report some good explanations. The DNA is stained blue so you should measure the size of the blue band.

Dr. Michelle

 

 


 Monitor Atmospheric Haze

 

 

AOT & spirit level

 

Hi Michelle,

Does the industrial waste in the atmosphere increases the AOT than a clear atmosphere?

When we measure the Sun intensity during the day, does the spirit level (LEVEL INDICATOR ON SCALE) should be at the center all the time?

Sundar

 

Re: AOT & spirit level

 

Sundar,

Typical AOT values are around 0.45 in polluted cities and 0.3 in rural areas.
Yes, the AOT is higher with with more pollution in the atmosphere.

The spirit level is only supposed to be used when you are taking a reading of the sun angle, not for a voltage reading.

Dr. Michelle

 


In our AOT formula, what does the constant 8660 represent

 

Hi Dr. Michelle,
In our AOT formula, what does the constant 8660 represent?
Kayla, my daughter, has completed the whole AOT analysis and is not sure of the constant "8660".

Let me know and regards.
Rolando

 

Re: In our AOT formula, what does the constant 8660 represent

 

As described on page 15, Mathematics of Monitoring Haze, the constant 8660 is an empirical factor that factors in Rayleigh scattering at the peak wavelength detected by your green LED (525 nm).

An empirical factor means that it is a number that has simply been measured from experiments, not derived from physics theory.

Rayleigh scattering is the scattering of light by tiny particles much smaller than the wavelength of the light, in this case mostly gas particles in the atmosphere. This scattering depends on the wavelength of the light.

The equation for the AOT that you are using is used to calculate how difficult it is for the sun's light to pass through the atmosphere at the wavelengths that your green LED detects, peaking at 525 nm. The part of the equation: MP/8660 takes into consideration the amount of Rayleigh scattered light between the sun and your device at those wavelengths detected by your LED.

Hope this helps,

Dr. Michelle


 

Measure the Interplanetary Magnetic Field


 

Data

 

1) What do you think about your science project?

My 12 y/o eventually learned a lot, but I had to dust off my physics and astronomy to help him understand what he was doing. I know that this was listed as 14+ on the web site, but he wanted to give it a try. The instructions say 12+, and he kept pointing this out to me when I would remind him of what he was tackling. Suggest you alter the printed instructions to say 14+.

2) Are you having problems understanding any of the diagrams or the instructions?

Our sensing magnet readily touched the CD case, impeding its rotation. It was not clear from the instructions that you needed to rotate the CD case until the magnet swings freely and orientates itself N/S, and that the nulling magnets need be oriented along the N/S axis. On our initial trials we had the CD case rotated perpendicular to N/S (!), so that the sensing magnet's rotation was limited by the CD case. (It was able to oscillate slightly, fooling us into thinking that we were detecting real IMF fluctuations.) Consequently, the nulling magnet had to be quite close (~10 cm) before the sensing magnet violently flipped around. We collected 2 days of data in which the device registered only random noise before I figured out what was wrong.

3) Do you have all the parts you need?

Missing 1 pair of nitrile gloves, could have used 3-4 pair (little boys make a mess)

4) What can do to improve this project?

A diagram showing the need to position the CD case to allow the sending magnet to rotate freely and attain N/S orientation, and that the nulling magnets need be positioned along the N/S axis passing through the sensing magnet, would have saved us a lot of time and frustration.

I'm not sure how good our responses were. The example showed wonderful correlation by an expert, presumably with rock steady table, collecting data in the same area as a USGA magnetometer. The Virginia site was the closest to us, at ~400 miles. I was not sure how much to attribute discrepancies to the distance, our device, etc.

An example of a typical tracing would help, particularly showing how much noise is created by simple vibrations from people living and moving about the house. Also comments on how much variation to expect when you are not near a USGS site.

We matched colors on the USGS site tracing to pick out the correct data. You provided the website for the L1 satellite data, but I could not tell which tracing to use from the lack of instruction documentation or web site documentation. Evan a comparison of graphs like the USGS example would have allowed me to match colors and graph notations.

5) What else can I do to make your science fair experience more successful and enjoyable?

I am attaching a Power Point slide with our data from the third day of data collection (the project was due the next day, so we were running short on time). Does this represent a reasonable result?

Thanks,
jtk

Attachments

lide2.jpg

Slide2.jpg (35.37 KiB) Viewed 640 times

lide4.jpg

Slide4.jpg (30.18 KiB) Viewed 638 times

lide3.jpg

 

Re: Data

Joseph,

Thank you so much for your detailed responses. We will take these into consideration for your next set of instructions. We have since changed our instructions to say "14+" It is a challenging project.

I did look at your data and it looks very good. So glad you were able to set up the webcam. You did see the same dip in the field that was seen at the USGS location but not the peak. It is true that a difference in 400 miles could explain some of the discrepancy. You can get a sense of this by comparing the data between the different USGS locations. Also, I'm wondering if their might have been something hindering the sensor magnet from swinging in that direction.

I think he did a great job and I hope you'll let us know how he does in the science fair.

Sincerely,

Dr. Michelle

 


 

Dependent and independent variables

 

Hi!

we ordered a science kit and will be taking the earth's magnetic pulse- however question for you is---
How would we show variables? Any help you could give us would be great!

Thanks for your time!
Eddie

Re: dependent and independent variables

 

Examples of possible variables are for example,

dependent: magnitude of interplanetary magnetic field from home build magnetometer; independent: magnitude of magnetic field as measured by the USGS professional ground instrument

dependent: magnitude of interplanetary magnetic field; independent: time of day

dependent: time delay in the interplanetary magnetic field of home built instrument and NASA's instrument; independent: each peak and valley measured

dependent: strength of the magnetic field, as measured by the deflection of the laser dot on your ruler on the wall (from the reflection on the sensor magnet); independent: distance of your test magnet from the sensor magnet

Hope this helps.

Dr. Michelle

 


Laser pointer position and magnitude of magnetic field

 

Dr. Shawn,

I got the magnetic pulse experiment kit, made the magnetometer, and recorded the results on the webcam for 7 hours in one day at one minute increments.
Your instructions were good, and It looks somewhat similar to the government's findings.
But how does the Y axis (laser pointer position and in video pixels) translate to numerical measurements of the magnetic field?

Also, in my science project, I must have a manipulated variable. I don't think I have one. What would it be?
I received your instructions on how to construct the magnetometer, but I did not get the "Complete Hypothesis to Conclusion Instructions" as mentioned in your website that I was suppose to receive.
Maybe those instructions would help to understand what my variable was.
Please email me back with instructions and advise on what to do.
Thank you-Joshua

 

Re: laser pointer position and magnitude of magnetic field

ostby DrMichelle on Mon Feb 23, 2009 3:38 pm

Hi Annette,

To get a numerical value for the field you have to calibrate the device using the calibration magnets that were included with the kit and the directions in the instructions. OR... you can compare your device to the USGS instrument. If that is calibrated, you can look at the same peak on your plot and their plot. The USGS data will tell how the strength of the magnetic field that produced that peak. The peak in your data will tell you how the displacement on your instrument that corresponds to that size magnetic field. Since your device has a linear response, you can easily calculate the magnetic field that corresponds to any displacement. If the peak displacement was produced by 40 nanogauss, then a displacement that was half as large would be correspond to a 20 nanogauss field and so on.

The manipulated variable... There is an independent variable--that's time. You are monitoring how the magnetic field changes in time. But you aren't manipulating the field. So there is no manipulated variable here. This is a problem with school science projects, the teachers don't understand how science gets done but they think they do. A great many scientific discoveries have been made by finding a new way to shin a light into a dimly lit room just to see what is there.

I suggest you show the teacher the results and discuss the need to have a manipulated variable under the circumstances. Your project clearly deserves a good grade and a chance to be judged by capable scientists.

One could measure the fall of with distance of the magnetic field of a small magnet and this is also explained in the text. Then you are manipulating a variable, the distance, and observing how the detector reacts. But this is so boring compared to connecting oneself directly to the solar wind.

I hope this helps you.

Yours for great science,

Dr. Shawn

 


 

Field of the dipole magnet

 

Hello, I'm back to bother you again.

1)The hypothesis my son picked was: "the field of the dipole magnet falls as the square of the distance". The problem is I have no clue what that means.

2) my son built the kit this weekend and has some questions:

a) as he pointed the beam onto the mirrored magnet, it reflected onto the wall (and the ruler) - he noticed it was not a perfect pinpoint dot reflection on the ruler - the reflection was vertical at the bottom and spread out at the top.
Is this normal? is he doing something wrong?

b) approximately 2 minutes after the beam was reflecting on the ruler, he noticed a shift from 15mm to 19mm for about 5 minutes - after that, it went back to 13mm.
Is this correct/ does this really happen?

c) if the shift is correct, how many Tesla's is it - neither he nor I have any idea how to measure

I will be home from 5:00 pm eastern at (305) 361-2882 and it would be really helpful if you or Dr. Shawn could call as both my son and I will be here.
I need to make sure he understands before going forward.
Thank you very much.

Noris

 

 

Re: field of the dipole magnet

 

Dear Noris,

1) To square a number means to multiply the number by itself. Consider 1 squared. (in email we write this as 1^2, with the ^ symbol meaning "raised to the power". In your son's report, he would write this as 1 followed by the numeral 2 superscripted. 1^2 = 1 since 1 x 1 = 1 Similarly...

2^2 = 4
3^2 = 9
4^2 = 16
5^2 = 25

and so on.

So to say that the force falls as the square of the distance means that if you double the distance between the two magnets, the force is only 1/4th as strong. If you triple the distance between the force between the magnets is just 1/9th as strong. If you increase the distance by a factor of 5, the force is only 1/25th as strong and so on. In other words the force (F) is proportional to 1 / r^2. When we say that the force falls as the distance squared, we mean that it follows relationship.

It's easy to test. Just measure the force at one distance, any distance you like. (In your case you can simply place the test magnet say 10 centimeters behind the magnetometer and measure the distance the spot moves along teh wall.) Then double that distance (move the magnet to 20 centimeters) and measure the force again. If the new force equals the first measurement divided by four, you know that the force falls as one of the square of the distance. That means, if the spot is only deflected one quarter the distance along the ruler.

But what happens if that's not what you find? Suppose you double the distance and the deflection is about 1/8th as much? Then the force doesn't follow the square law. It must be following some other law. Consider this

2 x 2 x 2 = 2^3 = 8
3^3 = 27
4^3 = 64

So, if the force falls as the distance cubed, then if you triple the distance the force will be only 1/27th as strong.

The way to do this experiment therefore is to measure the deflection for say ten distances from say 10 centimeters to 50 centimeters. If you assume the square law force, and you measure the deflection at say 10 centimeters, then it's easy to predict what the force should be at each of the other distances. The equation is the following.

deflection = deflection at 10 centimeters x (10 centimeters / distance)^2.

In words, for each measurement you make at some distance, you divide 10 by the distance, then square the result. Then you multiply that result by whatever deflection you measured at when the test magnet was 10 centimeters away from the sense magnets. You can then plot these numbers on a graph, and then connect the lines with a smooth curve. Did your data fall along that curve? If so, then you have proven that the force between two magnets falls as the square of the distance between them,

What if the deflections do not follow the curve? Then you need to try another power law. I suggest you try the cube law. Again, if you measure the deflection at one distance you can predict what the deflection should be at all other distances. The equation is almost exactly the same as the last one, except you cube the ratio of the distances instead of square it. In other words:

deflection = deflection at 10 centimeters x ( 10 centimeters / distance)^3

BTW: In your report, write the equation this way: D = D(10 cm) x (10 cm / d)^3 The D(10 cm) is shorthand for "deflection measured at 10 centimeters." This is the way a scientist would write it. And also you make sure that you measures all distances in the same units, in this case centimeters.

If you plot the points given by this equation you can see whether your actual data matches the predicted data. If they do, you've proven that the magnetic force actually falls off as 1 / r^3.

2) a) He is doing nothing wrong. The plastic mirror is not a perfect mirror, so it does distort the spot. What matters is that you identify some feature on the spot, (a dark area, a point of light or whatever) and always measure the distance that that particular part of the spot moves.

b) YES!!! If you've zeroed the detector correctly, you are watching real shifts in the earth's magnetic field. Most of these will be caused by the solar wind smashing into the ionosphere.

c) You have to determine for yourself how many Teslas it is because the sense magnets are all a little different. It will be in the range (assuming you have zeroed your detector properly) of 10 nano-Tesla, or 10 billionths of a Tesla. That's a fantastically tiny shift in the field!

I hope this helps clarify things. I can't call tonight because I have previous appointments. If you still have questions, please let me know.

Yours for great science,

Dr. Shawn

 

 


Build Your Own Cloud Chamber

 

 

Help understanding theory of cloud chamber

 

Hello,
My name is Monica and I am 6th grade student. My science fair is on Feb.
19, 2009. I purchased the cloud chamber project from Dr. Shawn's science
projects. I need some help understanding this project on a 6th grade
level. I have pulled several things from the web but for some reason, and
maybe it's me making it harder to understand than it is, I just can't
seem to get started on my project report. Doing the actual project, I
think will be no problem - explaining the theory and having a good solid
understanding of it is what I need help on. If you could please help me I
would be forever greatful.

Thanks so very much,
Monica

Re: help understanding theory of cloud chamber

 

Monica,

This is what I think you need to know at the most basic level. All matter is made up of atoms which contain protons and neutrons in the nucleus and electrons orbiting on the outside. Protons are positively charged, neutrons are neutral, and electrons are negatively charged. Under normal circumstances, atoms are neutral because they have an equal number of protons and electrons.

However, there is something called ionizing radiation that is all around us. It is simply any particle or wave that is energetic enough to strip electrons from atoms making them no longer neutral. Some examples of ionizing radiation are muons, beta particles, gamma rays, or X rays. I do not think it's important that you know what all these are at your level. What's important is that you know that they all are capable of stripping electrons from atoms making them no longer neutral but charged. When they are no longer neutral they are called ions. Also you should know that ionizing radiation can come from many different sources, from outer space, from the ground, or from radioactive sources like Uranium and is constantly around us. Only we can't see it.

The purpose of your cloud chamber is to make this ionizing radiation visible. In your cloud chamber what you do is create a very dense cloud of alcohol. The whole chamber is filled with alcohol but only where it is really dense can you see it because it has condensed into droplets large enough for us to see. It's the same with water vapor in the sky. It's all over the place but only where it is really dense does it form droplets that we can see.

Now, when some ionizing radiation zips through your cloud chamber, as it will because it's always around us, it will strip away the electrons from some of the atoms inside the chamber creating a trail of ions where the radiation zipped through. This line of ions then interacts with the alcohol inside your cloud chamber basically attracting a whole bunch of alcohol atoms towards the ions. When this happens, the alcohol atoms become dense enough to form droplets and be seen by us.

So, when you look inside your chamber in the area just below the alcohol cloud where the alcohol is not yet dense enough to be visible, you should be able to see little ghostly cloud tracks or squiggly trails where the ionizing radiation has zipped through and caused the alcohol to become dense enough to form droplet trails.

I hope this is helpful to you. Let me know if you have any further questions.

Sincerely,

Dr Michelle

 

 


distinguishing different particles

 

Hello,

We purchased the download on the cloud chamber (order 137111896) for our son's science fair project and are slightly confused. We are not scientists, but my husband and I couldn't tell by reading the information how to distinguish the presence of radon from the radiation particles mentioned in the article. Are we missing something, or did we misunderstand the scope of information we received?

Thanks for your help. This is our son's first science fair and we want him to enjoy the experience.

Ann

Re: distinguishing different particles

 

Hi Ann,

Radon is an alpha emitter and alphas are heavy, consisting of two protons and two neutrons. Therefore they'll react strongly inside the chamber and produce short thick tracks. Most other radioactive sources like muons produce beta particles instead which are high speed electrons or positrons and will go all the way through the chamber in thin slender tracks. Hope this helps.

Sincerely,

Dr. Michelle

 


Viewing an Ant's Brain

 

How do I take pictures of the insect parts

 

Dr. Shawn

My son wants to do the ant brain science fair project. Please let me know how I take pictures of the insects parts and how strong a microscope he needs. How do you take pictures of what you see in the microscope?

Sue
Jordan's Mom
5th grade student 10 years old

 

Re: How do I take pictures of the insect parts

 

Dear Sue,

The secret is simple. Just put the camera against the lens and snap away. If you take pictures using a digital camera, do so on the highest resolution. Read the images into your computer and blow them up to a reasonable size.

If the eye piece is too small to be able to get much of an image this way, then try the following. Remove the eye piece. This will have two lenses in it--the one you look through (the eye lens) and the one at the bottom. Unscrew the eye lens and set it aside. Next, tape a small piece of masking tape to the side of the eye piece. Now, turn the eye piece upside down back into the microscope. The tape will thicken the side and allow you to snug the eye piece back into place. The lens that was on the bottom of the eye piece is now on the top. That lens is much larger than the eye piece and is a whole lot easier to see through. If you hold the camera above the eye piece you should be able to focus through the lens and get great pictures of what's on the slide.

As to power, any microscope you find will work perfectly well.

I hope this helps you.

Yours for great science,

Dr. Shawn

 

 


 

EKG PROJECT

 

leads for the data-logger

 

Dear Dr. Shawn

I had a couple of questions about the download for the ecg. I was wondering where to connect the leads for the data-logger for one. I would think that the red lead would be connected to the output of the ecg, but where should the black lead be connected to? Should it just be connected to the ground or where? I was also wondering what should the potentiometer be adjusted to?
Thank you for your time.

James Swigert

 

Re: leads for the data-logger

 

Dear James,

The red lead goes to the output of the ECG. As you suspect, the black lead goes to the ground connection. The variable resistor is a zero-adjust. Adjusting the resistor will move the output voltage up or down. So watch the trace as you adjust the resistor and set it so that the trace is centered around zero voltage.

We definitely should explain that in the instructions. Thanks for letting me know that this needs improvement.

Take care,

Dr. Michelle

 


-9 volt lead

 

Dear Dr. Michelle

My son is doing the homemade ecg for his science fair and I had a questions about the schematic. On the diagram it shows that you apply a +9v after the the low pass filter. I was wondering where the -9v lead comes into play to finish that part of the circuit?
Thank you for your time.

James

 

Re: -9 volt lead

 

It doesn't. The -9V is only used to power the chip.

Dr. Michelle